Bio 1406 Enzyme Report

1) ABSTRACT The purpose of this sample was to carry forth the make of enzyme concentration, temperature, pH level, and an inhibitor on a type of enzyme called Perixodase. The purpose of the try was also to understand the relationship between the change in absorbance with time and the enzyme reaction direct. To perform the experiment, the class was divided into groups. sepa ordainly group took on one of the factors affecting the enzyme. Before halt the actual experiment, we apply the mixing tables that were given to us to step forwardline up the test tubes with the appropriate substances. We followed the same procedure for separately experiment variable (1-5). We then rid the cell of toxic hydrogen peroxide (H2O2) so the cell would not enclose damaged, as stated in our lab manual. For every observational variable, test tube 1 was always the control. Once the solutions were carry we mixed them and poured them into the cuvette and employ the spectrophotomete r machine to run the enzyme and determine its rate and its absorbance. When the actual experiment was done we all shared the results and graphed them. 2) macrocosm Before beginning the actual experiment we had to become long-familiar with a spectrometer machine and its functions.

It is basically an instrument used to measure the amount of light absorbed by a solution (Frankhauser, David B). As stated in our lab manual, the darker the solution, the greater its absorbance. The reason why we used this machine was to figure out the relationship between the change in absorbance with time and the enzyme reactio n rate. The enzyme reaction rate was determi! ned when bizs of absorbance versus time were constructed. The slope of all(prenominal) plot was related to the reaction rate. After running a subatomic experiment to learn how to use the spectrophotometer, we learned or so the background information of enzymes. Enzymes are proteins that serve as gas pedal (Ophardt, Charles E.). By definition, a catalyst is a substance that initiates or accelerates...If you want to get a full essay, order it on our website: OrderCustomPaper.com

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